d*****s
发帖数: 647 | 1 Nature. 2013 Nov 7;503(7474):146. doi: 10.1038/nature12727.
Retraction: Functional dissection of lysine deacetylases reveals that HDAC1
and p300 regulate AMPK.
Yu-yi Lin, Samara Kiihl, Yasir Suhail, Shang-Yun Liu, Yi-hsuan Chou, Zheng
Kuang, Jin-ying Lu, Chin Ni Khor, Chi-Long Lin, Joel S. Bader, Rafael
Irizarry & Jef D. Boeke
Nature 482, 251'255 (2012); doi:10.1038/nature10804
In response to a concern raised by a reader about inconsistencies in our
Letter between the results from the primary microarray screen and cell
growth validation studies (Supplementary Table 2), we reviewed the methods
described, and the subsequent analytical and validation work. We conclude
that the Methods section in our Letter is inaccurate, and that for 38% of
the interactions found by the primary screen there was a discordance in sign
when validated (for example, an interaction might be called 'synthetic
lethal' by the primary screen but 'synthetic rescue' in the cell growth
validation assay, or vice versa). Although the results from the reanalysis
did not contradict our key conclusions, we also re-evaluated the experiments
in Figs 2-4 that indicate that p300 acetylates PRKAA1 and HDAC1
deacetylates it. We sequence-verified and spot-checked many of the plasmids
and cell lines described, which are available on request. The biological
materials were found to be as we described, and certain aspects, such as
p300 acetylation of PRKAA1 protein in vitro, could be reproduced. However,
despite several attempts, we were unable to obtain results definitively
supporting the major claims of the Letter, namely that p300 is the
acetyltransferase and HDAC1 is the deacetylase for PRKAA1 in cells. The
batch of polyclonal antibody against acetyl-lysine originally used is no
longer available. More definitive experiments require antibodies that
specifically recognize acetylated sites on PRKAA1, which we are developing.
Although our inability to reproduce these results does not mean our
conclusions are incorrect, we cannot say with confidence that they are
correct. Given the time that has elapsed, and our inability to reproduce the
main conclusions of the Letter, we feel obliged to retract it. All the
authors have signed this retractionwith the exception of Y.-Y.L., who is
deceased. Correspondence should be addressed to J.D.B. ([email protected]
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/* ]]> */
). | s*********y
发帖数: 292 | 2 who is deceased....
HDAC1
【在 d*****s 的大作中提到】
: Nature. 2013 Nov 7;503(7474):146. doi: 10.1038/nature12727.
: Retraction: Functional dissection of lysine deacetylases reveals that HDAC1
: and p300 regulate AMPK.
: Yu-yi Lin, Samara Kiihl, Yasir Suhail, Shang-Yun Liu, Yi-hsuan Chou, Zheng
: Kuang, Jin-ying Lu, Chin Ni Khor, Chi-Long Lin, Joel S. Bader, Rafael
: Irizarry & Jef D. Boeke
: Nature 482, 251'255 (2012); doi:10.1038/nature10804
: In response to a concern raised by a reader about inconsistencies in our
: Letter between the results from the primary microarray screen and cell
: growth validation studies (Supplementary Table 2), we reviewed the methods
| s*******1
发帖数: 188 | 3 how about the corresponding author?
Will he/ she be punished? | m*********D
发帖数: 1727 | 4 Disputed Research Ends in Tragedy
A biomedical researcher whose Nature paper was called into question was
found dead in his lab.
By Dan Cossins | March 14, 2013
4 CommentsPrint
Link thisStumbleTweet this
FLICKR, PLAXCO LAB
A Taiwanese biomedical researcher who co-authored a Nature paper while at
Johns Hopkins University (JHU) may have killed himself last summer, on the
day he was due to submit a written response to questions regarding the study
's conclusions, reported The Washington Post.
The story centers on Daniel Yuan, formerly a statistician in the genetics
lab of Jef Boeke at JHU Medical School, and Yu-yi Lin, then a researcher in
the same lab. For years, Yuan had raised concerns about the methodology and
results of the lab’s studies on interactions between genes in yeast. To him
, the data didn’t always support the conclusions being drawn.
According to the Post, Yuan was first demoted and then dismissed in December
2011. By that time, Yuan already had serious concerns about data presented
in a study that was eventually published in Nature in February 2012, and he
later wrote to the journal’s editors to express his concerns that results
were overstated, and that he had seen no evidence that the analysis claimed
in the work had even been carried out.
In response, Nature’s editors had asked Boeke and lead author Lin, who had
since moved on to take a new position at Taiwan National University, to
submit a response to Yuan’s criticisms. But a day after Boeke wrote to
Nature to ask for more time, Lin was found dead in his lab in Taiwan,
surrounded by needles, sedatives, and muscle relaxants. Police ruled out
suicide because there was no suicide note at the scene. Lin's family told
the Taipei Times that he may have injected muscle relaxants due to work-
related pressure.
Within hours of the news of Lin’s death, a note was sent from Lin’s e-mail
address to the account of Yuan. “Yu-yi passed away this morning,” the e-
mail read. “You must be very satisfied with your success.”
Although he was shocked by the whole affair, Yuan told the Post that he then
asked Nature to publish his criticisms and inquired about Boeke’s response
. On September 28, a Nature editor told Yuan that they were waiting on a
proper response, but that “experiments are being done and probably a
Correction written.” That correction has yet to appear, though a
spokesperson for JHU told the Post that it was now under review by the
journal. As a last resort, Yuan also contacted the Office of Research
Integrity, who told him that the case is being investigated by JHU.
Correction (March 14): Yu-yi Lin's death was not reported as a suicide by
local news outlets, as previously stated. Lin was found dead in his lab,
surrounded by needles, sedatives, and muscle relaxants. Police ruled out
suicide because there was no suicide note at the scene. The Scientist
regrets the error.
Correction (March 15): The paper in question was not alleged to contain
false or fabricated data, as previously stated. Instead, Daniel Yuan
questioned the methods used to analyze the data and the conclusions
subsequently drawn. The headline, subhed, and lede of the article have been
changed to reflect this. The Scientist regrets the error. | T****a
发帖数: 409 | | P*A
发帖数: 7996 | | s*****j
发帖数: 6435 | 7 统计害死人呀.
【在 T****a 的大作中提到】
: Sigh~~~
| s*********y
发帖数: 292 | 8 额，竟然真的是自杀……
study
【在 m*********D 的大作中提到】
: Disputed Research Ends in Tragedy
: A biomedical researcher whose Nature paper was called into question was
: found dead in his lab.
: By Dan Cossins | March 14, 2013
: 4 CommentsPrint
: Link thisStumbleTweet this
: FLICKR, PLAXCO LAB
: A Taiwanese biomedical researcher who co-authored a Nature paper while at
: Johns Hopkins University (JHU) may have killed himself last summer, on the
: day he was due to submit a written response to questions regarding the study
| x******h
发帖数: 838 | 9 这就是那个著名的千老自杀老板当院士的事情啊。
【在 s*********y 的大作中提到】
: 额，竟然真的是自杀……
:
: study
| m***i
发帖数: 2013 | | | | s*****g
发帖数: 7857 | 11 好死不如赖活着。
自杀不可取。
大不了不干科研了。
【在 m***i 的大作中提到】
: 很惨烈。不知道是不是又一个内斗的案例
| j******n
发帖数: 1727 | 12 同组的两个千老内斗。。。
【在 m***i 的大作中提到】
: 很惨烈。不知道是不是又一个内斗的案例
| R*****D
发帖数: 153 | 13 几年前重复这个组发明的一种yeast genetic interaction方法。大概花了八九个月时
间，每次实验可重复性极差，最后不得不放弃一个基于该方法的 project。
。。。
。。。。。。
这个事件严重了影响我的生涯。真是极其鄙视大牛发灌水文章，害人匪浅。 | R****E
发帖数: 391 | 14 学术界的歪风早该杀一杀了
HDAC1
【在 d*****s 的大作中提到】
: Nature. 2013 Nov 7;503(7474):146. doi: 10.1038/nature12727.
: Retraction: Functional dissection of lysine deacetylases reveals that HDAC1
: and p300 regulate AMPK.
: Yu-yi Lin, Samara Kiihl, Yasir Suhail, Shang-Yun Liu, Yi-hsuan Chou, Zheng
: Kuang, Jin-ying Lu, Chin Ni Khor, Chi-Long Lin, Joel S. Bader, Rafael
: Irizarry & Jef D. Boeke
: Nature 482, 251'255 (2012); doi:10.1038/nature10804
: In response to a concern raised by a reader about inconsistencies in our
: Letter between the results from the primary microarray screen and cell
: growth validation studies (Supplementary Table 2), we reviewed the methods
| c***y
发帖数: 615 | 15 Lin is the corresponding author...
【在 s*******1 的大作中提到】
: how about the corresponding author?
: Will he/ she be punished?
| r******8
发帖数: 1486 | 16 笑死，very true
【在 P*A 的大作中提到】
: 赚白菜钱的比卖白粉的伤亡率还高的时代快来了
|
|
