y****6 发帖数: 196 | 1 A nice article that might help to explain your weird microscopic data.
Immunolabeling artifacts and the need for live-cell imaging
Ulrike Schnell,
Freark Dijk,
Klaas A Sjollema
& Ben N G Giepmans
Affiliations
Corresponding author
Nature Methods 9,152–158(2012)doi:10.1038/nmeth.1855Published online 30
January 2012 | s*****j 发帖数: 6435 | 2 hehe, 看来对co-localization的文章得越来越小心了.
【在 y****6 的大作中提到】 : A nice article that might help to explain your weird microscopic data. : Immunolabeling artifacts and the need for live-cell imaging : Ulrike Schnell, : Freark Dijk, : Klaas A Sjollema : & Ben N G Giepmans : Affiliations : Corresponding author : Nature Methods 9,152–158(2012)doi:10.1038/nmeth.1855Published online 30 : January 2012
| l**********1 发帖数: 5204 | 3 Oui
Before 3D or 4D 5D ND STED/PALM dream comes Truth,
do not trust those below roughly 180 nm scale GFP/YFP fusion protein microscopy picture.
New Book Chapter 1:
HTTPS://openaccess.leidenuniv.nl/bitstream/handle/1887/17922/vanDriel-H1-
Introduction.pdf?sequence=3
Ps:
Princeton PI Sara said one half month ago:
//www.mitbbs.com/article_t/Biology/31628031.html
【在 s*****j 的大作中提到】 : hehe, 看来对co-localization的文章得越来越小心了.
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