d*********e
发帖数: 18 | 1 通过gel shift assay (EMSA)计算了蛋白跟一系列底物的结合常数kd.
但是审稿人说：EMSA measurements are non-equilibrium and do not yield Kd
values.
请教应该如何回应？
有些文献用表观解离常数kapp来替代kd，这个是否正确？
谢谢。 | b******y
发帖数: 627 | 2 I agree with the referee. But does the absolute Kd matter in your case or
the relative Kd? For example, you want to demonstrate wt and mutant proteins
have a big difference in binding the same ligand. In either case, to a
reasonable referee, you might be OK if using Kapp instead of Kd in the paper
and also acknowledge your agreement with his/her opinion in the rebuttal
letter. Good luck! | d*********e
发帖数: 18 | 3 谢谢回复。
我只是想比较同一蛋白对不同底物的结合能力。Kapp应该可以说明问题。
proteins
paper
【在 b******y 的大作中提到】
: I agree with the referee. But does the absolute Kd matter in your case or
: the relative Kd? For example, you want to demonstrate wt and mutant proteins
: have a big difference in binding the same ligand. In either case, to a
: reasonable referee, you might be OK if using Kapp instead of Kd in the paper
: and also acknowledge your agreement with his/her opinion in the rebuttal
: letter. Good luck!
| b******y
发帖数: 627 | |
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