y*********u
发帖数: 183 | 1 Early sign of chimera mice that carrying gremlin transmission.
Dear all, I am in a hurry to decide if it is worth to inject another ES cell
line of a KO mice.
The ES cell line I am using is SV129 cell line ,which carrying agouti coat
colour.
Right now I am having 9 male chimera mice and 1 female chimera mice, all of
the male chimera mice are with very high agouti number indicating a strong
ES cell contribution, and also, as stated above, a distorted gender ratio.
Meantime , I am getting above 80% of normal karyotype
Is there any early sign we can count on to determine the possibility of ES
cell germ line contribution?
Some people told me that regardless how good the early sign is, it is still
possible to have all the male mice failed to give out germline transmission
, which is very disturbing. |
y*********u
发帖数: 183 | |
m******5
发帖数: 1383 | |
i*********0
发帖数: 915 | 4 normally we inject several clones and then backcross to b6 and wait and
confirm with southern or PCR. |
m******5
发帖数: 1383 | 5 不知道楼主的情况。。我是因为冰箱事故只有一个克隆活下来
【在 i*********0 的大作中提到】
: normally we inject several clones and then backcross to b6 and wait and
: confirm with southern or PCR.
|
y*********u
发帖数: 183 | 6 我们也没好到哪里去，８个克隆只有一个ｋａｒｙｏｔｙｐｅ正常，７个是７号ｔｒｉ
ｓｏｍｙ
【在 m******5 的大作中提到】
: 不知道楼主的情况。。我是因为冰箱事故只有一个克隆活下来
|
m******5
发帖数: 1383 | 7 你们是不是一开始挑了特别大的克隆……
【在 y*********u 的大作中提到】
: 我们也没好到哪里去，８个克隆只有一个ｋａｒｙｏｔｙｐｅ正常，７个是７号ｔｒｉ
: ｓｏｍｙ
|
m******5
发帖数: 1383 | 8 理论上说那种特别大的colony很有可能是trisomy 8
【在 m******5 的大作中提到】
: 你们是不是一开始挑了特别大的克隆……
|
m******5
发帖数: 1383 | |
