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发帖数: 70 | 1 i have never worked with plant, so i do not know whether this
could be due to sth that cuase higher obsorbance at 260nm.
the ratio for isolated RNA from animal tissues is usually 1.7-
2.0 for my experience, and it pretty much depends upon what
kind of kit/reagent i use. i try TRIZol (Invitrogen), which
is pretty good, but sometimes RNA from pancreas will degrade
with no mercy. i also use RNeasy Kit (Qiagen), which is said
to be better, particularly working for microarray purpose.
i would recomme |
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