M****e
发帖数: 70 | 1 is there IP assay available? i think it is necessary if you
can fractionate the protein by columns (many biochemcal stuffs
that i am not familiar, which i read from purification of
p300 transcription factor, i think people in this area knows
a lot). then do the assay to see which fraction has the
activity (is this specific reaction?) thus you know the effective
size.
is this endogenous or expressed protein? guess it is
endogenous, given the big size. try IP with the antibody and
do the assay. or |
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