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发帖数: 129 | 1 The method to make transgenic mice is by microinjection of
your construct DNA into the pronucleus of the fertilized
oocyte. Your construct will integrate into the chromosome
randomly, and usually tandemly in a single chromosome. The
amount of expression is dependent on the copy numbers of
your favorite gene, and also the promoter you choose
Depending on the purpose of your research, you can choose
tissue-specific or ubiquitous promoter. Sometimes, you just
want to use its own promoter. In your c |
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