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全部话题 - 话题: fragments
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d**********o
发帖数: 1321
1
来自主题: WebRadio版 - 潜水员冒泡兼征版友意见
第一个项目report
这时偶刚到CSAC工作不久,与小A同学还不熟,我用的还是latex。随着贴的作业越来越
多,应该是用有共同爱好的小伙伴更亲密些。这次贴latex,下次才再org-mode。
\documentclass[b5paper,11pt, abstraction, titlepage]{scrartcl}
\usepackage[utf8]{inputenc}
\usepackage[T1]{fontenc}
\usepackage{CJKutf8}
\usepackage{multirow}
\usepackage{multicol}
\usepackage{listings}
\usepackage{geometry}
\geometry{b5paper}
\usepackage{graphicx,floatrow}
\usepackage{graphicx,subfigure}
\newsavebox{\abstractbox}
\renewenvironment{abstract}
{\begin{lrbox}{0}\begin{minipage}{\t... 阅读全帖
d**********o
发帖数: 1321
2
来自主题: WebRadio版 - 潜水员冒泡兼征版友意见
第一个项目report
这时偶刚到CSAC工作不久,与小A同学还不熟,我用的还是latex。随着贴的作业越来越
多,应该是用有共同爱好的小伙伴更亲密些。这次贴latex,下次才再org-mode。
\documentclass[b5paper,11pt, abstraction, titlepage]{scrartcl}
\usepackage[utf8]{inputenc}
\usepackage[T1]{fontenc}
\usepackage{CJKutf8}
\usepackage{multirow}
\usepackage{multicol}
\usepackage{listings}
\usepackage{geometry}
\geometry{b5paper}
\usepackage{graphicx,floatrow}
\usepackage{graphicx,subfigure}
\newsavebox{\abstractbox}
\renewenvironment{abstract}
{\begin{lrbox}{0}\begin{minipage}{\t... 阅读全帖
Y**u
发帖数: 5466
3
来自主题: Wisdom版 - The True Meditation
I hope you can all hear me, can you? Is it quite audible? Right. This is the
last talk. During the last three times that we have met here we have been
talking about the chaos in the world, the misery, the suffering, and the
desire for entertainment and the avoidance of fear. We went into several
things concerning our daily life. During all these talks it must have been
very clear that we are concerned with the human transformation
psychologically, for that alone can bring about a change in the s... 阅读全帖
C*I
发帖数: 4736
4
一直在误导,现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人,必须
经过一个中间宿体性的其它动物才能传染给人。所以,病毒发生后,就故意误导全国人
民去海鲜市场找证据,找其它野生动物的麻烦。 而且还是病毒所去找的,找完了还装
模做样化验呀,分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
,一直不敢说,说了其它相关已经在人就会去早那种动物监测。 所以压根不说,打马
虎眼。
事实上,早在2013 年,就是这个武汉病毒研究所,已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒,其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体,并能够感
染人的细胞。对此新发现,武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”,以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说,从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus, 可以不经过
其它受体/宿体,而直接传染给人。 他们... 阅读全帖
C*I
发帖数: 4736
5
一直在误导,现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人,必须
经过一个中间宿体性的其它动物才能传染给人。所以,病毒发生后,就故意误导全国人
民去海鲜市场找证据,找其它野生动物的麻烦。 而且还是病毒所去找的,找完了还装
模做样化验呀,分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
,一直不敢说,说了其它相关已经在人就会去早那种动物监测。 所以压根不说,打马
虎眼。
事实上,早在2013 年,就是这个武汉病毒研究所,已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒,其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体,并能够感
染人的细胞。对此新发现,武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”,以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说,从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus, 可以不经过
其它受体/宿体,而直接传染给人。 他们... 阅读全帖
C*I
发帖数: 4736
6
Published: 30 October 2013
Isolation and characterization of a bat SARS-like coronavirus that uses the
ACE2 receptor
Xing-Yi Ge, Jia-Lu Li, Xing-Lou Yang, Aleksei A. Chmura, Guangjian Zhu,
Jonathan H. Epstein, Jonna K. Mazet, Ben Hu, Wei Zhang, Cheng Peng, Yu-Ji
Zhang, Chu-Ming Luo, Bing Tan, Ning Wang, Yan Zhu, Gary Crameri, Shu-Yi
Zhang, Lin-Fa Wang, Peter Daszak & Zheng-Li Shi
Nature volume 503, pages535–538(2013)Cite this article
Abstract
The 2002–3 pandemic caused by severe acute respirator... 阅读全帖
W***u
发帖数: 789
7
来自主题: Seattle版 - 昨天天上掉下了大陨石!
07:19 AM ET
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Comments (167 comments)
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Rush on to find fragments of California meteor
There's a new rush on in California's gold rush country. This time, they're
prospecting for meteorites.
A minivan-sized meteor blew up over northern California on Sunday morning,
and now everyone from NASA scientists to schoolkids is looking for fragments
of the fireball – called meteorites once they hit the ground – in the... 阅读全帖
r*****t
发帖数: 4793
8
来自主题: Biology版 - paper help!
Nature. 2012 Mar 14;483(7390):434-8. doi: 10.1038/nature10895.
Intrinsic coupling of lagging-strand synthesis to chromatin assembly.
Smith DJ, Whitehouse I.
Source
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York
Avenue, New York, New York 10065, USA.
Abstract
Fifty per cent of the genome is discontinuously replicated on the lagging
strand as Okazaki fragments. Eukaryotic Okazaki fragments remain poorly
characterized and, because nucleosomes are rapidly deposited on n... 阅读全帖
h******d
发帖数: 4761
9
http://www.handloads.com/misc/stoppingpower.asp?Caliber=2&Weigh
22 Long Rifle Stopping Power, all bullet weights
Brand Bullet Shootings One Shot Stops Percent Diameter
Penetration Notes
Quik Shok HP 10 4 40% 0" 6.7"
CCI Stinger HP 465 178 38% 0.28" 7.3"
Winchester HP 654 196 30% 0.29" 9.6"
Federal HP 722 214 30% 0.26" 8.8"
Remington HP 988 296 29% 0.25" 9.1"
Winchest... 阅读全帖
w********g
发帖数: 447
10
来自主题: Chemistry版 - 做Mass Spectrometry 的大牛请进
从你的mass来看,唯一的两个可能就是
C8H12O4和C4H8N6O2。
如果看fragmentation,所有的fragment按照N规则,似乎都是不含N的,要所有的
fragment都不含N,那么分子本身含N的可能性比较小。也就是说第二个的可能性比较小
。如果你的isotopic peak的ratio是准的话,和第二个分子式match,这样又排除了第
一个。而且第二个分子式算出来的mass和你测得的只差1.7个ppm,对于FTMS来说比较
make sense。而第一个分子式的计算值和你测得要差17.3个ppm,对于FTMS来说有点太
大了。
感觉分子式C4H8N6O2,但是回到开始说的N规则,如果有6个N的话,你所有的
fragmentation的碎片里面either要含有所有的6个N,或者每次掉偶数个N。另外看你的
fragmentation,碎片几乎都分布在比较靠近molecular ion的一端,低mass几乎看不到
太多的fragmentation,感觉最大的可能是你的6个N都不太容易掉,比如都长在几个共
轭的环上,环上有些支链。但你又说是个直链的东西,那可能是一些直链
g**********n
发帖数: 4
11
工作描述如下, 本人的背景跟这个位置非常match,简历基本cover所有discription,
已经在线投简历,还请有关前辈多多指点,帮忙再push一下简历给Hiring Manager就太
好了!先谢了.
(在线简历是个德文网站,还要browser翻译成英文,最离谱的是还提示要用“原始语
言”投简历,以便recruiter筛选,算是奇葩经历了)
Job Description A health care company with global reach. A product pipeline
filled to the brim. A team committed to scientific advancement. Think what's
possible. Novartis and its associated companies are always looking for
talented employees globally. We are engaged in advance preparation for
potential position openings.... 阅读全帖
t*******r
发帖数: 3271
12
来自主题: EmergingNetworking版 - 求面试问题
Describe the series of events going on when the 4 Routers connected the
Ethernet power up the same time.
What fields are contained in OSPF’s HELLO Packets ? (10 40 seconds)
Desribe Hello Packets when two routers are in the 2WAY state (neighbor field
) …
Can a router with a higher priority pre-empt a DR or BDR ?
What does Priority 0 mean ?
Describe the various LSA types (1,2,3,4,5) and what they are used for ?
1. Explain the 1st line – what is is (NET)
2. What’s in the second line ? (LSP-ID)
Expl... 阅读全帖
i**i
发帖数: 1500
13
http://readwrite.com/2014/08/14/google-android-open-soure
把苹果的命给革了以后,却发现革命群众不是那么好带的.
Never has a tweet been more true, or potentially more disastrous for a
vendor. Years ago Google's Andy Rubin, stung by Steve Jobs' criticism that
Android wasn't truly open, tweeted that anyone could fork - i.e., modify -
Android, making it "open" in the truest sense of the word.
Unfortunately for Google, many OEMs took Rubin at his word.
Today, Google's Android business is booming, but it's clear that Android
fr... 阅读全帖
d**p
发帖数: 149
14
来自主题: Biology版 - 问个cloning 的问题
Can you find the restriction enzyme site less than 2kb distance from the
insertion site? If yes, you do not have amplify very long fragments. Similar
size of fragments can joint together easier than different size fragments.
Another way: You can amplify the whole plasmid( How big is the plasmid?) by
the primers at the insertion site. Then you can introduce the enzyme site in
the primer. After PCR amplify the plasmid, digest it and ligate your 800bp
fragment and transform. You need to amplify you... 阅读全帖
s********n
发帖数: 2939
15
来自主题: Biology版 - 实例:利用NGS检测治病菌
现在这种例子越来越多了
http://www.nytimes.com/2014/06/05/health/in-first-quick-dna-tes
In a First, Test of DNA Finds Root of Illness
Joshua Osborn, 14, lay in a coma at American Family Children’s Hospital in
Madison, Wis. For weeks his brain had been swelling with fluid, and a
battery of tests had failed to reveal the cause.
The doctors told his parents, Clark and Julie, that they wanted to run one
more test with an experimental new technology. Scientists would search
Joshua’s cerebrospinal fluid for piece... 阅读全帖
g*****x
发帖数: 3283
16
来自主题: Biology版 - NGS vs mass spectrometry
bottom-up proteomics和拼图类似,先把peptide送进collision chamber,因为
fragmentation有一定的pattern,所以产生的abcxyz fragment ion可以和从database
sequence中计算预测的fragment ion进行比较:能match的fragment越多,对这个
peptide的confidence就越高。
目前主流仪器对单个peptide的fragments能cover八九成,但在较复杂的体系中对不同
peptide的coverage会下降,这个主要还是仪器扫描速度,频率和通量等的限制,并不
是因为database本身太小的缘故。
A********e
发帖数: 354
17
【 以下文字转载自 Biology 讨论区 】
发信人: albertsmwk (.)(.), 信区: Biology
标 题: 第一批“青年千人计划”生物类@publication列表@
发信站: BBS 未名空间站 (Thu Aug 18 15:04:16 2011, 美东)
花了一个小时,深深的鄙视一下自己的无聊行径!!
125 蔡亮 男 1980年11月 复旦大学 生命
科学 2007年12月毕业于[美国]北卡大学 [美国]加州大学旧金山分校 博士后
Cai L, Mostov K. Polarity is destiny. Cell. 2009 Nov 13;139(4):660-2. PubMed
PMID: 19914162; PubMed Central PMCID: PMC2900917.
Cai L, Makhov AM, Schafer DA, Bear JE. Coronin 1B antagonizes cortactin and
remodels Arp2/3-containing actin branche... 阅读全帖
p*******g
发帖数: 2976
18
【 以下文字转载自 Biology 讨论区 】
发信人: albertsmwk (.)(.), 信区: Biology
标 题: 第一批“青年千人计划”生物类@publication列表@
发信站: BBS 未名空间站 (Thu Aug 18 15:04:16 2011, 美东)
花了一个小时,深深的鄙视一下自己的无聊行径!!
125 蔡亮 男 1980年11月 复旦大学 生命
科学 2007年12月毕业于[美国]北卡大学 [美国]加州大学旧金山分校 博士后
Cai L, Mostov K. Polarity is destiny. Cell. 2009 Nov 13;139(4):660-2. PubMed
PMID: 19914162; PubMed Central PMCID: PMC2900917.
Cai L, Makhov AM, Schafer DA, Bear JE. Coronin 1B antagonizes cortactin and
remodels Arp2/3-containing actin branche... 阅读全帖
O***n
发帖数: 13127
19
【 以下文字转载自 Biology 讨论区 】
发信人: albertsmwk (.)(.), 信区: Biology
标 题: 第一批“青年千人计划”生物类@publication列表@
发信站: BBS 未名空间站 (Thu Aug 18 15:04:16 2011, 美东)
花了一个小时,深深的鄙视一下自己的无聊行径!!
125 蔡亮 男 1980年11月 复旦大学 生命
科学 2007年12月毕业于[美国]北卡大学 [美国]加州大学旧金山分校 博士后
Cai L, Mostov K. Polarity is destiny. Cell. 2009 Nov 13;139(4):660-2. PubMed
PMID: 19914162; PubMed Central PMCID: PMC2900917.
Cai L, Makhov AM, Schafer DA, Bear JE. Coronin 1B antagonizes cortactin and
remodels Arp2/3-containing actin branche... 阅读全帖
f*********n
发帖数: 11154
20
来自主题: GunsAndGears版 - 5种不同的.38子弹测试(ZZ)
http://handgunsmag.com/ammunition/loads_for_snub_nose_revolvers_111610/index.html
......
However, velocity loss is quite significant with a two-inch barrel, and as
velocity diminishes, bullet expansion decreases and penetration increases.
Therefore, the most important question for the pocket revolver owner is,
Just how good, terminally speaking, is the fodder occupying the cylinder?
......
The test platform was a Crimson Trace laser grip-equipped S&W Model 49
Bodyguard. I engaged one m.o.a. dots... 阅读全帖
a**e
发帖数: 8800
21
禁掉铅子弹,子弹涨价
非铅子弹导致枪管磨损加大,导致枪成本增加。
。。。。。。
In November, one of the oldest condors in Central California died from lead
poisoning after being found with tiny bullet fragments and a .22-caliber
slug in his gullet that he apparently swallowed with a mouthful of meat.
The 9-year-old giant was one of the earliest released in a condor recovery
program along the Big Sur coast. His death -- and the recent death of a
golden eagle near Sacramento -- are being highlighted by health and
environmental groups wh... 阅读全帖
l*****a
发帖数: 38403
22
......
Christian Origin Theory
While there are certainly some common characteristics shared between
different Jewish sectarian groups, most
scholars deny that there is any connection between the Christians and the
authors of the Dead Sea Scrolls. There is
little to suggest that the Christians at this time were as secluded or as
interested in elaborate rituals of purity and
chastity as the community that produced the Scrolls. Still, Spanish Jesuit
Josep O'Callaghan-Martínez has argued that
one fr... 阅读全帖
S***k
发帖数: 370
23
来自主题: Database版 - sql server 面试题 (4)
Shrink database or shrink files is to remove unused pages and release disk
space. It is usually used after delete operations, such as truncate a table
or drop a table.In most cases, the fragmentation related to performance is
index fragmentation which is the internal or external fragmentation of the
pages supporting indexes. Because removing unused pages could not help
reduce the degree of index pages fragmentation, shrink could not help too
much to improving performance.
Repeatedly shrinking a
n***h
发帖数: 11
24
来自主题: Biology版 - Re: Yeast two hybrid system?
照我的记忆说来:
yeast 2 hybrid system是两个蛋白之间的binding
yeast 1 hybrid是蛋白质和DNA之间的binding ,就象ras说的,测出的是bait和
fragment之间的结合,可以象ras说的那样fragment是library, 来找和已知蛋白
结合的特定element,也可以特定的fragment,转cDNA library进去,找和这个
fragment 结合的蛋白。 (infact that's what I did)
回来说2hybrid。transcriptional factor一般至少有两个domain: binding domain
和activition domain. binding domain binds to DNA elements, while activation
domain activates the transcription of downstream genes. 我们要测试蛋白A
和蛋白B是否bind, 就做以下两个construct:
—————————— —————————
R*s
发帖数: 2041
25
来自主题: Biology版 - Re: Yeast two hybrid system?
example:
--------------------- ----------------------------
| bait | activator| + |fragment A | reporter gene |
--------------------- ----------------------------
Bait ait is the gene u r intestered in, and is attached to a
transcription activator. fragment A is one of those from a DNA library
which is attached to a reporter gene.
Basically you transfect both the bait construct and library construct
into yeast (a and alpha respectively) and let the mate (each yes... 阅读全帖
n***h
发帖数: 11
26
来自主题: Biology版 - Re: Yeast two hybrid system?
照我的记忆说来:
yeast 2 hybrid system是两个蛋白之间的binding
yeast 1 hybrid是蛋白质和DNA之间的binding ,就象ras说的,测出的是bait和
fragment之间的结合,可以象ras说的那样fragment是library, 来找和已知蛋白
结合的特定element,也可以特定的fragment,转cDNA library进去,找和这个
fragment 结合的蛋白。 (infact that's what I did)
回来说2hybrid。transcriptional factor一般至少有两个domain: binding domain
和activition domain. binding domain binds to DNA elements, while activation
domain activates the transcription of downstream genes. 我们要测试蛋白A
和蛋白B是否bind, 就做以下两个construct:
—————————— —————————... 阅读全帖
j*****9
发帖数: 716
27
http://scienceblogs.com/webeasties/2010/12/guest_post_arsenate-based_dna.php
In the wake of the NASA excitement over the new arsenic study, and my
promise to give a detailed review of the paper itself, I have recruited a
colleague with strong opinons about the work, a solid chemistry and
microbiology background, and "Dr." in front of his name to share his
analysis. I will be posting have posted my personal and less-technical take
on the whole thing soon, so stay tuned as well.
Dr. Alex Bradley u... 阅读全帖
L******e
发帖数: 679
28
I am going to use SOLiD system.
There is two libraries i am going to prepare:
1), Regular fragmentation, 150 bp / fragment, then link to adaptors.
2), Mate-paired: link two ends of a fragment (1-2 kb) together. (i had
thought that is why also called 'paired-end') .
According to your description, pair-ends is the regular fragments, but why
was call 'pair-ends', is that because it was sequenced from two ends?
Thanks
m***i
发帖数: 898
29
来自主题: Biology版 - 问个IP/ID的实验技术问题
WB检测到新的protein bands,位置在55kd和28kd,设想是目标蛋白的fragments, 想用
同一抗体IP这两个fragments, 然后再用mass spectrometry ID证实是目标蛋白的
fragments。
但目前IP后fragments的信号被igG heavy and light chains所屏蔽(both for IB and
MS).大家有什么办法可以去除IgG的信号?谢谢
C*******e
发帖数: 4348
30
hoho,我这两天做的也是基于pUC19的
是pUC19(SacI)-(SacI)fragment I(XmaI)-(XmaI)fragment II(BamHI)-(BamHI)
fragment III(XbaI)-(XbaI)fragmentIV(HindIII)-pUC19(HindIII)
每个fragment大概0.8-1.1kb
然后我做的是pUC19 50 ng,
pUC19:f1:f2:f3:f4=1:5:5:5
总体积15-20 ul
ligate 20 hrs
然后3 ul加到一管TOP10电感化态细胞里
涂100-200 ul/平板
然后挑克隆
用colony digestion筛选
一起做了五个ligation
一天筛了近90个克隆
每个ligation都筛出正确的了
然后这些positive的提质粒出来
用不同的酶切再确认一下
然后测个序(或者不测)
直接PCR的切就行
不过我个人比较喜欢的是每个片段先做到TOPO里面(pBluntII)
先测序
挑序列正确的再酶切、胶纯化,往下做
这样每一步都是正确的序列
最后终产物酶切什么都对的话不测序也没关系
不然如果... 阅读全帖
a********k
发帖数: 2273
31
花了一个小时,深深的鄙视一下自己的无聊行径!!
125 蔡亮 男 1980年11月 复旦大学 生命
科学 2007年12月毕业于[美国]北卡大学 [美国]加州大学旧金山分校 博士后
Cai L, Mostov K. Polarity is destiny. Cell. 2009 Nov 13;139(4):660-2. PubMed
PMID: 19914162; PubMed Central PMCID: PMC2900917.
Cai L, Makhov AM, Schafer DA, Bear JE. Coronin 1B antagonizes cortactin and
remodels Arp2/3-containing actin branches in lamellipodia. Cell. 2008 Sep
5;134(5):828-42. PubMed PMID: 18775315; PubMed Central PMCID: PMC2570342.
Cai L, Makhov AM, Bear JE. F-actin... 阅读全帖
C******2
发帖数: 97
32

vectors
The construction is modified and derived from pRetrosuper which is used for
random mutation in host genome based on gene-trap. Truncated 3’LTR
fragment is promoter activaty lost. The idea is host genome will be mutated
by insertion because the existence of splicing acceptor(SA) fragment thus
generate a fusion RNA containing cherry CDS. Fusion florescence protein can
be detected in some case when insertion occurs as an in frame manner. The
insertion will also make the infected cells be... 阅读全帖
K******S
发帖数: 10109
33
ETD sensitivity/efficiency比CID低是肯定的
CID在ION TRAP里还是最快的
FUSION叫TRIBRID是你可以同时做3件事情.我觉得如何利用这个优势更重要.比如在
ORBITRAP做MS1或者HCD的时候别让ION TRAP闲着.
CID FRAGMENT的时候是M/Z FOCUS的,意味着如果PEPTIDE上的修饰容易掉的话,就不会有
很好的BACKBONE FRAGMENTS (因为修饰掉了M/Z就变了), ETD可以解决这个. 但现在有
HCD了,是QUADRUPOLE FRAGMENTATION (修饰掉了还可以继续FRAGMENTATION),对修饰的
PEPTIDE效果也不错.
尤其FUSION的速度太快了,15HZ ORBITRAP, 20HZ ION TRAP. 那这个当QE使唤有些大材
小用了.
k****o
发帖数: 728
34
如果你的peptide比较长,以至于产生很多+3--+5甚至更高的fragment ions,那么无论
对于ETD还是CID,在OT里做high resolution的MS2都更有利。而ETD是有利于high
charge state,long peptide的fragmentation。所以,如果你的样品确实有很多long
peptide的话(你去raw data file,average一段MS1的data就有底了),用OT scan
MS2会让搜索效果更好。你搜索时记得把fragment ions的error window舍得很窄,这样
才起到high resolution的效果。比如10 or 20ppm,或者0.01 m/z,取决于你具体的
accuracy。你可以试试MS2用15000 resolution with 20 ppm error window。
关于省时间的问题。诚然IT确实比OT省时间,如果你用老一代的LTQ Orbitrap,这个确
实是个大concern。但技术在进步,Orbitrap Fusion已经在设计上提供了比过去快得多
的ion transmi... 阅读全帖
p*******g
发帖数: 2976
35
【 以下文字转载自 Biology 讨论区 】
发信人: albertsmwk (.)(.), 信区: Biology
标 题: 第一批“青年千人计划”生物类@publication列表@
发信站: BBS 未名空间站 (Thu Aug 18 15:04:16 2011, 美东)
花了一个小时,深深的鄙视一下自己的无聊行径!!
125 蔡亮 男 1980年11月 复旦大学 生命
科学 2007年12月毕业于[美国]北卡大学 [美国]加州大学旧金山分校 博士后
Cai L, Mostov K. Polarity is destiny. Cell. 2009 Nov 13;139(4):660-2. PubMed
PMID: 19914162; PubMed Central PMCID: PMC2900917.
Cai L, Makhov AM, Schafer DA, Bear JE. Coronin 1B antagonizes cortactin and
remodels Arp2/3-containing actin branche... 阅读全帖
z*******n
发帖数: 1034
36
More
Posted May 7, 2014 at 11:30 pm by Will Verduzco
[APK] Google Play Services 4.4 Brings Street View API, Activity Recognition,
Game Services Update, Wallet Fragments, and More
Today, we’ve seen a spattering of first-party Android application updates.
We first were given a nice update to the Google Camera application, which
restored the ability to take still snapshots while recording video. We then
saw several minor application updates to Wallet, Search, YouTube, Docs,
Sheets, and Cloud Print.... 阅读全帖
w*******y
发帖数: 60932
37
In case anyone missed previous deals.
Link:
http://www.bestbuy.com/site/olstemplatemapper.jsp?_dyncharset=ISO-8859-1&id=pcat17071&type=page&ks=960&st=Video_Games_Fallout_New_Vegas_Sale_20101216&sc=Global&cp=1&sp=-bestsellingsort skuid&qp=&list=n&usc=All Categories&nrp=15&p=[promotion, synonymns]&_D:p= &pu=defaultusr&_D:pu= &pt=1292479201&iht=n&_DARGS=/site/en_US/search/fragments/includes/olssearchparameters.jsp.frmSearchResults
#91;promotion,+synonymns]&_D:p=+&pu=defaultusr&_D:pu=+&pt=1292479201... 阅读全帖
M*****8
发帖数: 17722
38
来自主题: Military版 - 隐形技术的血泪源头。(ZT)
隐形技术的血泪源头。(ZT)
The tearful origins of China's stealth
By Peter Lee
The recent test flight of China's J-20 stealth fighter has occasioned
certain uproar in international security circles, as well as paroxysms of
joy among China's more nationalistic netizens.
Despite no hard information on its stealthiness or its capabilities beyond
the fact that it was able to take off, fly for 15 minutes, and land, the J-
20 is already serving as justification for heightened concern and its
inevitable adjunct, h... 阅读全帖
w*********g
发帖数: 30882
39
笔者对此发表以下看法:
(1)卫生部2007年颁布实施《新资源食品管理办法》有意取消了2002年颁布实施
的《转基因食品卫生管理办法》“第五条 转基因食品的食用安全性和营养质量不得低
于对应的原有食品”这个条款,是退步,而不是进步!这种删除的实质是,进一步顺从
邪恶孟山都为首跨国转基因产业的私利与压力,置中国人民持续安全、健康、生存与繁
衍于不顾!
(2)卫生部2007年颁布实施《新资源食品管理办法》有意取消了2002年颁布实施
的《转基因食品卫生管理办法》“第五条”,但是卫生部《新资源食品管理办法》“第
三条”规定:
第三条 新资源食品应当符合《食品卫生法》及有关法规、规章、标准的规定,对
人体不得产生任何急性、亚急性、慢性或其他潜在性健康危害。
依据该第三条规定,在对于抗草甘膦转基因大豆加工的转基因大豆油,检测出超过
俄罗斯对大豆油草甘膦残留量允许标准与中国卫生部对棉籽油草甘膦残留最高限量2.9-
6倍的草甘膦残留量,已经证实对人类健康,特别对青少年、儿童、婴儿与孕妇及其胎
儿,造成极其严重危害情况下,(1)对人类健康不是形成 “潜在性健康危害”而是... 阅读全帖
l******t
发帖数: 55733
40
来自主题: Military版 - 找到碎片了
Vietnam Searchers Report Spotting Plane Debris
Officials Say They Found Piece of Door, Airplane Tail
Email
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↓ More
52 Comments
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By JASON NG, GAURAV RAGHUVANSHI and JAKE MAXWELL WATTS CONNECT
Updated March 9, 2014 9:59 a.m. ET
The fate of Malaysia Airlines flight MH370 is still unknown and the search
continues. The WSJ's Jake Maxwell Watts tells us the focus remains in the
South China Sea between Malaysia and Vietnam.
KUALA LUMPUR—A Vietna... 阅读全帖
m***e
发帖数: 428
41
来自主题: Faculty版 - 华人社会里的西方社会科学
三 困难之本体论方面的根源
华人学生赖以成长的社会,在制度和文化的各方面与西方社会均有着重要的历史的和现
状的差异,因为这一系列存在经验的差异而对诸多现代西方社会科学的概念不易接受,
是笔者所观察到的最普遍的一种困难。
例一。在西方社会科学文献里,bureaucracy是一个中心概念。对当代bureaucracy讨论
影响最大的,是韦伯(Max Weber)的著名定义:
The following may thus be said to be the fundamental categories of rational
legal authority:
(1)A continuous organization of official functions bound by rules.
(2)A specified sphere of competence…
(3)The organization of offices follows the principle of hierarchy…
(4)The rules which regulate the conduct of an off... 阅读全帖
q********g
发帖数: 10694
42
Molecular Modeling and Organic Synthesis Opportunity
Sr. Scientist, Molecular Modeler and Organic Synthetic Chemist
MindPharm is partnering with an established BioPharmaceutical company to
identify candidates for an exciting Functional Imaging program designed to
augment Drug Development and Biomarker Discovery.
Primary Function:
Independently conceive, execute and communicate novel multi-disciplinary
research or development strategies that achieve project and area goals.
Generate new scientific... 阅读全帖
S**********s
发帖数: 4534
43
我觉得medium profile加不加fluting都正好。
初速方面,看你准备用啥弹药. 小口径高初速弹讲究fragmentation, m855的reliable/
minimum fragmentation初速是2700-2500fps
16寸枪管, m855弹的fragmentation距离是大概100/150米,
14.5寸枪管则是50/100米。
14.5如果想可靠的让m855弹形成破片, 距离必须在50米内...这有点悲剧, 如果用
Hornady 75gr TAP, 破片距离是2250fps, 也就是175m
16寸枪管打75gr TAP则是200m
结论, SHTF只能大量囤积普通弹药, 所以要用16寸。
z*****y
发帖数: 142
44
以下来自FAMI通访谈:
SE鸟山求:TBC的意味在于游戏结尾后还有很多后续内容,比如碎片结局,后续DLC等,所以没有用系列以往一贯用的“END”。
问:前传小说《THE FRAGMENTS BEFORE》最后也是TBC(TBC IN THE FRAGMENTS AFTER)呢。
SE鸟山求答:在后续的外传小说《THE FRAGMENTS AFTER》里会描写游戏中没有
交代的同时期发生的事情,比如比如解释雪叔怎样又变成路西等等。
问:游戏里打了几瓶酱油的黑人父子,详情完全不明了呢。
SE鸟山求:确实阿。比如黑人父子和鸟娘等事件(注:这三个是有关系的),目前在考虑是出DLC呢还是出小说等来补完。
以下来自美版攻略书访谈:
问:雪叔为什么能穿越?他手上的路西印记是怎么回事?
SE渡边大佑:雪叔终于相信了赛拉的想法,于是独自寻找L姐。后来他找到并主动接近了一个法尔希,希望能从它身上得到穿越的能力,后来他就变成了路西并找到了一个门,于是就开始了他漫长的旅程,这段会在小说里描写。而接下来的故事在游戏里以文字的形式详细地表述了(英雄的轨迹)。
问:黑人父子怎么能穿越?为什么他们突然出现在游戏结尾... 阅读全帖
m*****8
发帖数: 1027
45
来自主题: TVGame版 - FF13_2 的隐藏结局太坑爹了吧
隐藏结局要收集全160个fragment,我第一次通关还不到50个fragment。
大部分的fragment都是透明的,不看攻略能找得到吗?即便收集全了,隐藏结局的动画也
只有1分钟,而且和普通结局没差别啊,goddness挂了,对吧。
再吐槽一下小游戏,转钟表这个太无耻了吧,backtrack算法解很合适,人脑跟不上吧。
智力问题也很无耻,庞大的题库,连续10道答对才有奖品。
游乐场的公鸡赛跑很有趣?
最后再吐槽下PS3的FF13_2的save editor,没有Xbox版的好,只能批量修改最大化属性值
m**y
发帖数: 18546
46
来自主题: Love版 - 贴歌吃包子(结束)
Ashes and Wine
http://www.youtube.com/watch?v=us818dZgdp8
Don't know what to do anymore
I've lost the only love worth fighting for
And I'll drown in my tears, don't they see?
And that would show you, that would make you hurt like me
All the same I don't want mudslinging games
It's just a shame to let you walk away
Is there a chance, a fragment of light
At the end of the tunnel, a reason to fight?
Is there a chance you may change your mind
Or are we ashes and wine?
Don't know if our fate's alread... 阅读全帖
o***1
发帖数: 592
47
来自主题: TrustInJesus版 - 多玛福音
多马福音(Gospel of Thoma'),又译多默福音或多玛斯福音,被认为是新约时代的一
本伪典,其完整的科普特语(Coptic)手抄版本於1945年在埃及的拿戈玛第 (Nag
Hammadi)镇被发现,采用一种今日称之为科普特钉装法的方式来钉装。它与正典的四福
音不同之处在於:它除了声称是由耶稣十二门徒之一的多马(天主教作「多默」)所写以
外,全卷只是记录了耶稣的言论,并没有提及耶稣的传道过程也没有记载耶稣受难复活
的经历。
当本经文的完整科普特语版本译出後,圣经考古学家立即意识到其中的文句对应於1896
年到1904年间在奥克西林库斯(Oxyrhynchus)出土的希腊文残页(即写在残破蒲草纸板
的希腊文版多马福音)。根据考古学家的推断,目前所收集到的希腊文手抄版本来自三
份抄本(P. Oxy. I 1; IV 654; IV 655),都是在公元200年初所抄写,而这份科普特
语手抄版本则成卷於公元340年左右。虽然科普特语版本的内容与希腊文的残本并不完
全相同,但相信它是从更早期的希腊文古文翻译而来的。
多马福音的文本在1975年向公众释出。从此,它被翻译成多国文字,并且出... 阅读全帖
b**********y
发帖数: 163
48
来自主题: BMU版 - 每日一题之生理生化
题目
pieces of dog parotid gland and of pancreas are transplanted under the skin of
the donor animal. after re-establishment of a circulation, ingestion of food
will result in secretion from:
a. parotid and pancreatic transplants.
b. the fragment of the parotid only.
c. the fragment of the pancreas only.
d. neither fragments.
which one?
a***y
发帖数: 19743
49
来自主题: Apple版 - [合集] 随着os4的退出
☆─────────────────────────────────────☆
ronaldinho10 (苹果,微软,狗狗) 于 (Thu Apr 8 21:58:49 2010, 美东) 提到:
不好意思,是推出 —— 我觉得爱愤在智能手机里至少又可以安心的坐稳两年。
☆─────────────────────────────────────☆
lint (take it easy) 于 (Thu Apr 8 22:01:13 2010, 美东) 提到:
光个os4不行,还是没android自由牛x,看iPhone4怎样了
希望是个真正的ultimate phone,偶也进个把玩把玩

☆─────────────────────────────────────☆
ronaldinho10 (苹果,微软,狗狗) 于 (Thu Apr 8 22:04:51 2010, 美东) 提到:
如果爱愤能装多系统就好了
☆─────────────────────────────────────☆
lint (take it easy) 于 (Th... 阅读全帖
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